Bisulfite sequencing is a collective name for a group of methods, aimed for DNA methylation pattern studies. Bisulfite treatment of DNA turns cytosine residues into uracil, without affecting 5-methylcytosine. After PCR amplification uracil is substituted by thymine, thus further analysis distinguishes single-nucleotide polymorphisms separating cytosines and thymines.
Comparison of the sequence obtained from the bisulfite-treated amplicon to the untreated one (published sequence) enables the identification of differential methylations.
Whole-genome methylation analysis.
|Genome size||Average coverage||Run time||Price per sample|
|3Gb||x30||6-12 weeks||Request a quote|
|2Gb||x30||6-12 weeks||Request a quote|